apc coupled igg antibody Search Results


anti tnf apc vio770 ca2  (Miltenyi Biotec)
98
Miltenyi Biotec anti tnf apc vio770 ca2
Anti Tnf Apc Vio770 Ca2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facscanto ii flow cytometer  (Becton Dickinson)
90
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dynabeads  (Thermo Fisher)
97
Thermo Fisher dynabeads
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anti arginase 1  (R&D Systems)
93
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af835  (R&D Systems)
96
R&D Systems af835
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mouse igg  (Synaptic Systems)
90
Synaptic Systems mouse igg
Mouse Igg, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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mouse monoclonal immunoglobulin type g proteins igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse monoclonal immunoglobulin type g proteins igg
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anti β actin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti β actin
Anti β Actin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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apc-cy7-anti-cd45  (Sony)
90
Sony apc-cy7-anti-cd45
Apc Cy7 Anti Cd45, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hrp coupled goat antimouse igg1 ab  (SouthernBiotech)
94
SouthernBiotech hrp coupled goat antimouse igg1 ab
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antimouse cd80  (SouthernBiotech)
93
SouthernBiotech antimouse cd80
Figure 2 APC inhibits maturation of bone marrow-derived DCs. Notes: Bone marrow cells were prepared from C57BL/6 mice, cultured in the presence of Flt3L, and then APC was added to the culture on day 6 and stimulated with LPS for 24 hours. The expression of MHC class II, CD86, and <t>CD80</t> on CD11c+ DCs in the presence of 40 µg/mL of APC (A) and the mean fluorescent intensity were measured (B). The effects of different doses of APC on maturation markers and secretion of cytokines from DCs (C) are also shown. Data are expressed as the mean ± standard error of the mean (n=3). The figure shows the representative result from one of three independent experiments. *P,0.05 compared to the untreated group. Abbreviations: APC, activated protein C; MHC, major histocompatibility complex; MFI, mean fluorescence intensity; IL, interleukin; DCs, dendritic cells; LPS, lipopolysaccharide; n, number.
Antimouse Cd80, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apc vio770 recombinant human anti b220  (Miltenyi Biotec)
95
Miltenyi Biotec apc vio770 recombinant human anti b220
KEY RESOURCES TABLE
Apc Vio770 Recombinant Human Anti B220, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2 APC inhibits maturation of bone marrow-derived DCs. Notes: Bone marrow cells were prepared from C57BL/6 mice, cultured in the presence of Flt3L, and then APC was added to the culture on day 6 and stimulated with LPS for 24 hours. The expression of MHC class II, CD86, and CD80 on CD11c+ DCs in the presence of 40 µg/mL of APC (A) and the mean fluorescent intensity were measured (B). The effects of different doses of APC on maturation markers and secretion of cytokines from DCs (C) are also shown. Data are expressed as the mean ± standard error of the mean (n=3). The figure shows the representative result from one of three independent experiments. *P,0.05 compared to the untreated group. Abbreviations: APC, activated protein C; MHC, major histocompatibility complex; MFI, mean fluorescence intensity; IL, interleukin; DCs, dendritic cells; LPS, lipopolysaccharide; n, number.

Journal: Journal of Asthma and Allergy

Article Title: Activated protein C modulates the proinflammatory activity of dendritic cells

doi: 10.2147/jaa.s75261

Figure Lengend Snippet: Figure 2 APC inhibits maturation of bone marrow-derived DCs. Notes: Bone marrow cells were prepared from C57BL/6 mice, cultured in the presence of Flt3L, and then APC was added to the culture on day 6 and stimulated with LPS for 24 hours. The expression of MHC class II, CD86, and CD80 on CD11c+ DCs in the presence of 40 µg/mL of APC (A) and the mean fluorescent intensity were measured (B). The effects of different doses of APC on maturation markers and secretion of cytokines from DCs (C) are also shown. Data are expressed as the mean ± standard error of the mean (n=3). The figure shows the representative result from one of three independent experiments. *P,0.05 compared to the untreated group. Abbreviations: APC, activated protein C; MHC, major histocompatibility complex; MFI, mean fluorescence intensity; IL, interleukin; DCs, dendritic cells; LPS, lipopolysaccharide; n, number.

Article Snippet: 14 2 on 1 0- N ov -2 01 8 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Journal of Asthma and Allergy 2015:8 submit your manuscript | www.dovepress.com Dovepress Dovepress 31 Dendritic cell modulation by APc with the anti-CD16/CD32 FcR block (rat IgG2b, clone: 2.4G2) and stained with antimouse CD11c (Armenian hamster IgG1, clone: HL3), antimouse CD11b (rat IgG2b, clone: M1/70), antimajor histocompatibility complex (MHC) II (I-A/I-E, rat IgG2b, clone: M5.114), antimouse CD86 (rat IgG2a, clone: GL-1), antimouse CD80 (rat IgG2a, clone: 1G10, Southern Biotechnology Associates, Inc., Birmingham, AL, USA), antimouse CD45R (rat IgG2a, clone: RA3-6B2), or their isotype controls.

Techniques: Derivative Assay, Cell Culture, Expressing, Immunopeptidomics, Fluorescence

Figure 3 APC inhibits the maturation of human monocyte-derived DCs. Notes: Monocytes were prepared from human peripheral blood and cultured in the presence of recombinant GM-CSF and IL-4, and then APC (40 µg/mL) was added to the culture on day 6 and stimulated with LPS for 24 hours. The expression of HLA- DR, CD86, and CD80 on CD11c+ DCs in the presence or absence of APC (A), as well as the secretion of TNFα from DCs (B) are shown. Data are expressed as the mean ± standard error of the mean. The figure shows the representative result from one of three independent experiments. *P,0.05 compared to the untreated group. Abbreviations: Max, maximum; APC, activated protein C; MFI, mean fluorescence intensity; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TNFα, tumor necrosis factor alpha; DCs, dendritic cells; GM-CSF, granulocyte macrophage colony-stimulating factor; IL, interleukin; LPS, lipopolysaccharide.

Journal: Journal of Asthma and Allergy

Article Title: Activated protein C modulates the proinflammatory activity of dendritic cells

doi: 10.2147/jaa.s75261

Figure Lengend Snippet: Figure 3 APC inhibits the maturation of human monocyte-derived DCs. Notes: Monocytes were prepared from human peripheral blood and cultured in the presence of recombinant GM-CSF and IL-4, and then APC (40 µg/mL) was added to the culture on day 6 and stimulated with LPS for 24 hours. The expression of HLA- DR, CD86, and CD80 on CD11c+ DCs in the presence or absence of APC (A), as well as the secretion of TNFα from DCs (B) are shown. Data are expressed as the mean ± standard error of the mean. The figure shows the representative result from one of three independent experiments. *P,0.05 compared to the untreated group. Abbreviations: Max, maximum; APC, activated protein C; MFI, mean fluorescence intensity; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TNFα, tumor necrosis factor alpha; DCs, dendritic cells; GM-CSF, granulocyte macrophage colony-stimulating factor; IL, interleukin; LPS, lipopolysaccharide.

Article Snippet: 14 2 on 1 0- N ov -2 01 8 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Journal of Asthma and Allergy 2015:8 submit your manuscript | www.dovepress.com Dovepress Dovepress 31 Dendritic cell modulation by APc with the anti-CD16/CD32 FcR block (rat IgG2b, clone: 2.4G2) and stained with antimouse CD11c (Armenian hamster IgG1, clone: HL3), antimouse CD11b (rat IgG2b, clone: M1/70), antimajor histocompatibility complex (MHC) II (I-A/I-E, rat IgG2b, clone: M5.114), antimouse CD86 (rat IgG2a, clone: GL-1), antimouse CD80 (rat IgG2a, clone: 1G10, Southern Biotechnology Associates, Inc., Birmingham, AL, USA), antimouse CD45R (rat IgG2a, clone: RA3-6B2), or their isotype controls.

Techniques: Derivative Assay, Cell Culture, Recombinant, Expressing, Fluorescence

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: APC-Vio770 Recombinant human anti B220(clone REA755) , Miltenyi Biotec , Cat# 130–110-849; RRID:AB_2658286.

Techniques: Negative Control, Recombinant, Purification, Staining, Gene Expression, Lysis, Immunoprecipitation, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Blocking Assay, Cell Isolation, Software, Microscopy